SARS antibody test for serosurveillance.
Identifieur interne : 005135 ( Main/Exploration ); précédent : 005134; suivant : 005136SARS antibody test for serosurveillance.
Auteurs : Po-Ren Hsueh [Taïwan] ; Chuan-Liang Kao ; Chun-Nan Lee ; Li-Kuan Chen ; Mei-Shang Ho ; Charles Sia ; Xin De Fang ; Shugene Lynn ; Tseng Yyuan Chang ; Shi Kau Liu ; Alan M. Walfield ; Chang Yi WangSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Virus du SRAS.
- sang : Anticorps antiviraux.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Flambées de maladies, Humains, Sensibilité et spécificité, Surveillance de la population, Test ELISA, Études rétrospectives.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Disease Outbreaks, Enzyme-Linked Immunosorbent Assay (methods), Humans, Population Surveillance (methods), Retrospective Studies, SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology).
- MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay, Population Surveillance.
- Disease Outbreaks, Humans, Retrospective Studies, Sensitivity and Specificity.
Abstract
A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.
DOI: 10.3201/eid1009.040101
PubMed: 15498156
Affiliations:
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Le document en format XML
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Walfield</name></author><author><name sortKey="Wang, Chang Yi" sort="Wang, Chang Yi" uniqKey="Wang C" first="Chang Yi" last="Wang">Chang Yi Wang</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Disease Outbreaks</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Population Surveillance (methods)</term><term>Retrospective Studies</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Flambées de maladies</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Surveillance de la population ()</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Test ELISA ()</term><term>Virus du SRAS (immunologie)</term><term>Études rétrospectives</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Population Surveillance</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>Humans</term><term>Retrospective Studies</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Flambées de maladies</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Surveillance de la population</term><term>Test ELISA</term><term>Études rétrospectives</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chang, Tseng Yyuan" sort="Chang, Tseng Yyuan" uniqKey="Chang T" first="Tseng Yyuan" last="Chang">Tseng Yyuan Chang</name><name sortKey="Chen, Li Kuan" sort="Chen, Li Kuan" uniqKey="Chen L" first="Li-Kuan" last="Chen">Li-Kuan Chen</name><name sortKey="Fang, Xin De" sort="Fang, Xin De" uniqKey="Fang X" first="Xin De" last="Fang">Xin De Fang</name><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name><name sortKey="Lee, Chun Nan" sort="Lee, Chun Nan" uniqKey="Lee C" first="Chun-Nan" last="Lee">Chun-Nan Lee</name><name sortKey="Liu, Shi Kau" sort="Liu, Shi Kau" uniqKey="Liu S" first="Shi Kau" last="Liu">Shi Kau Liu</name><name sortKey="Lynn, Shugene" sort="Lynn, Shugene" uniqKey="Lynn S" first="Shugene" last="Lynn">Shugene Lynn</name><name sortKey="Sia, Charles" sort="Sia, Charles" uniqKey="Sia C" first="Charles" last="Sia">Charles Sia</name><name sortKey="Walfield, Alan M" sort="Walfield, Alan M" uniqKey="Walfield A" first="Alan M" last="Walfield">Alan M. Walfield</name><name sortKey="Wang, Chang Yi" sort="Wang, Chang Yi" uniqKey="Wang C" first="Chang Yi" last="Wang">Chang Yi Wang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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